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trap5b activity  (Immunodiagnostic Systems)

 
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    Immunodiagnostic Systems trap5b activity
    A Representative fluorescence images of the osteoclast phagocytosis assay in bone marrow–derived macrophages. BMM were stimulated with macrophage colony-stimulating factor (M-CSF), apoptotic thymocytes (APO), and receptor activator of nuclear factor kappa-B ligand (RANKL). Apoptotic thymocytes were labeled with CFDA (green), cytoplasm with CellMask Orange (orange), and nuclei with Hoechst 33342 (blue). Separate channels and merged overlays are shown. Cells with two nuclei were classified as pre-osteoclasts, and cells with three or more nuclei as osteoclasts. B Relative changes in the number, perimeter, and area of multinucleated TRAP+ cells after differentiation, with or without APO and transforming growth factor beta1 (TGF-β1) inhibitor. C Representative images of tartrate-resistant acid phosphatase (TRAP)-stained osteoclast cultures. D <t>TRAP5b</t> and E TGF-β protein expression in the supernatant of osteoclast cultures. F Analysis of relative gene expression of Nfatc1 (major transcription factor in osteoclastogenesis) , Acp5 (TRAP critical for osteoclastogenesis), and Rank (a receptor specific to osteoclasts and progenitors) gene. Statistical analysis involved a one-way ANOVA followed by Tukey’s multiple post hoc test. Data are presented as mean ± SD, and significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001 ( n = 3–8).
    Trap5b Activity, supplied by Immunodiagnostic Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap5b+activity/trap5b/pmc12847818-152-11-15
    Average 86 stars, based on 1 article reviews
    trap5b activity - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "Disrupted bone microenvironment and immune recovery following total body irradiation in a murine model"

    Article Title: Disrupted bone microenvironment and immune recovery following total body irradiation in a murine model

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-025-08303-7

    A Representative fluorescence images of the osteoclast phagocytosis assay in bone marrow–derived macrophages. BMM were stimulated with macrophage colony-stimulating factor (M-CSF), apoptotic thymocytes (APO), and receptor activator of nuclear factor kappa-B ligand (RANKL). Apoptotic thymocytes were labeled with CFDA (green), cytoplasm with CellMask Orange (orange), and nuclei with Hoechst 33342 (blue). Separate channels and merged overlays are shown. Cells with two nuclei were classified as pre-osteoclasts, and cells with three or more nuclei as osteoclasts. B Relative changes in the number, perimeter, and area of multinucleated TRAP+ cells after differentiation, with or without APO and transforming growth factor beta1 (TGF-β1) inhibitor. C Representative images of tartrate-resistant acid phosphatase (TRAP)-stained osteoclast cultures. D TRAP5b and E TGF-β protein expression in the supernatant of osteoclast cultures. F Analysis of relative gene expression of Nfatc1 (major transcription factor in osteoclastogenesis) , Acp5 (TRAP critical for osteoclastogenesis), and Rank (a receptor specific to osteoclasts and progenitors) gene. Statistical analysis involved a one-way ANOVA followed by Tukey’s multiple post hoc test. Data are presented as mean ± SD, and significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001 ( n = 3–8).
    Figure Legend Snippet: A Representative fluorescence images of the osteoclast phagocytosis assay in bone marrow–derived macrophages. BMM were stimulated with macrophage colony-stimulating factor (M-CSF), apoptotic thymocytes (APO), and receptor activator of nuclear factor kappa-B ligand (RANKL). Apoptotic thymocytes were labeled with CFDA (green), cytoplasm with CellMask Orange (orange), and nuclei with Hoechst 33342 (blue). Separate channels and merged overlays are shown. Cells with two nuclei were classified as pre-osteoclasts, and cells with three or more nuclei as osteoclasts. B Relative changes in the number, perimeter, and area of multinucleated TRAP+ cells after differentiation, with or without APO and transforming growth factor beta1 (TGF-β1) inhibitor. C Representative images of tartrate-resistant acid phosphatase (TRAP)-stained osteoclast cultures. D TRAP5b and E TGF-β protein expression in the supernatant of osteoclast cultures. F Analysis of relative gene expression of Nfatc1 (major transcription factor in osteoclastogenesis) , Acp5 (TRAP critical for osteoclastogenesis), and Rank (a receptor specific to osteoclasts and progenitors) gene. Statistical analysis involved a one-way ANOVA followed by Tukey’s multiple post hoc test. Data are presented as mean ± SD, and significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001 ( n = 3–8).

    Techniques Used: Fluorescence, Phagocytosis Assay, Derivative Assay, Labeling, Staining, Expressing, Gene Expression

    Related Articles

    Expressing:

    Article Title: Disrupted bone microenvironment and immune recovery following total body irradiation in a murine model
    Article Snippet: .. After 3 days of cultivation, TGF-β1 protein expression (Invitrogen 88-50690-22) and TRAP5b activity (Bone TRAP, Immunodiagnostic Systems) were measured in the culture supernatant from BMM differentiated osteoclasts. .. To study gene expression, RNA was extracted from cells lysed in RLT buffer using the RNeasy Micro QIAcube Kit (Qiagen, Hilden, Germany).

    Activity Assay:

    Article Title: Disrupted bone microenvironment and immune recovery following total body irradiation in a murine model
    Article Snippet: .. After 3 days of cultivation, TGF-β1 protein expression (Invitrogen 88-50690-22) and TRAP5b activity (Bone TRAP, Immunodiagnostic Systems) were measured in the culture supernatant from BMM differentiated osteoclasts. .. To study gene expression, RNA was extracted from cells lysed in RLT buffer using the RNeasy Micro QIAcube Kit (Qiagen, Hilden, Germany).



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    Immunodiagnostic Systems trap5b activity
    A Representative fluorescence images of the osteoclast phagocytosis assay in bone marrow–derived macrophages. BMM were stimulated with macrophage colony-stimulating factor (M-CSF), apoptotic thymocytes (APO), and receptor activator of nuclear factor kappa-B ligand (RANKL). Apoptotic thymocytes were labeled with CFDA (green), cytoplasm with CellMask Orange (orange), and nuclei with Hoechst 33342 (blue). Separate channels and merged overlays are shown. Cells with two nuclei were classified as pre-osteoclasts, and cells with three or more nuclei as osteoclasts. B Relative changes in the number, perimeter, and area of multinucleated TRAP+ cells after differentiation, with or without APO and transforming growth factor beta1 (TGF-β1) inhibitor. C Representative images of tartrate-resistant acid phosphatase (TRAP)-stained osteoclast cultures. D <t>TRAP5b</t> and E TGF-β protein expression in the supernatant of osteoclast cultures. F Analysis of relative gene expression of Nfatc1 (major transcription factor in osteoclastogenesis) , Acp5 (TRAP critical for osteoclastogenesis), and Rank (a receptor specific to osteoclasts and progenitors) gene. Statistical analysis involved a one-way ANOVA followed by Tukey’s multiple post hoc test. Data are presented as mean ± SD, and significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001 ( n = 3–8).
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    MiR‐106b inhibition decreased osteoclast numbers and suppressed osteoclast formation. A, Representative slices with TRAP staining. Scale bar: 50 μm. B, Oc.S/BS and C, N.Oc/BS were calculated. D, Representative immunohistochemical images of CTSK and MMP‐9. Scar bar: 100 μm. E,F, Semi‐quantitative analysis of CTSK and MMP‐9 expression. G, Serum levels of CTX and H, <t>TRAP5b</t> in each group were determined. n = 6 per group. * P < .05, ** P < .01, *** P < .001
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    MiR‐106b inhibition decreased osteoclast numbers and suppressed osteoclast formation. A, Representative slices with TRAP staining. Scale bar: 50 μm. B, Oc.S/BS and C, N.Oc/BS were calculated. D, Representative immunohistochemical images of CTSK and MMP‐9. Scar bar: 100 μm. E,F, Semi‐quantitative analysis of CTSK and MMP‐9 expression. G, Serum levels of CTX and H, <t>TRAP5b</t> in each group were determined. n = 6 per group. * P < .05, ** P < .01, *** P < .001
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    MiR‐106b inhibition decreased osteoclast numbers and suppressed osteoclast formation. A, Representative slices with TRAP staining. Scale bar: 50 μm. B, Oc.S/BS and C, N.Oc/BS were calculated. D, Representative immunohistochemical images of CTSK and MMP‐9. Scar bar: 100 μm. E,F, Semi‐quantitative analysis of CTSK and MMP‐9 expression. G, Serum levels of CTX and H, <t>TRAP5b</t> in each group were determined. n = 6 per group. * P < .05, ** P < .01, *** P < .001
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    Image Search Results


    A Representative fluorescence images of the osteoclast phagocytosis assay in bone marrow–derived macrophages. BMM were stimulated with macrophage colony-stimulating factor (M-CSF), apoptotic thymocytes (APO), and receptor activator of nuclear factor kappa-B ligand (RANKL). Apoptotic thymocytes were labeled with CFDA (green), cytoplasm with CellMask Orange (orange), and nuclei with Hoechst 33342 (blue). Separate channels and merged overlays are shown. Cells with two nuclei were classified as pre-osteoclasts, and cells with three or more nuclei as osteoclasts. B Relative changes in the number, perimeter, and area of multinucleated TRAP+ cells after differentiation, with or without APO and transforming growth factor beta1 (TGF-β1) inhibitor. C Representative images of tartrate-resistant acid phosphatase (TRAP)-stained osteoclast cultures. D TRAP5b and E TGF-β protein expression in the supernatant of osteoclast cultures. F Analysis of relative gene expression of Nfatc1 (major transcription factor in osteoclastogenesis) , Acp5 (TRAP critical for osteoclastogenesis), and Rank (a receptor specific to osteoclasts and progenitors) gene. Statistical analysis involved a one-way ANOVA followed by Tukey’s multiple post hoc test. Data are presented as mean ± SD, and significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001 ( n = 3–8).

    Journal: Cell Death & Disease

    Article Title: Disrupted bone microenvironment and immune recovery following total body irradiation in a murine model

    doi: 10.1038/s41419-025-08303-7

    Figure Lengend Snippet: A Representative fluorescence images of the osteoclast phagocytosis assay in bone marrow–derived macrophages. BMM were stimulated with macrophage colony-stimulating factor (M-CSF), apoptotic thymocytes (APO), and receptor activator of nuclear factor kappa-B ligand (RANKL). Apoptotic thymocytes were labeled with CFDA (green), cytoplasm with CellMask Orange (orange), and nuclei with Hoechst 33342 (blue). Separate channels and merged overlays are shown. Cells with two nuclei were classified as pre-osteoclasts, and cells with three or more nuclei as osteoclasts. B Relative changes in the number, perimeter, and area of multinucleated TRAP+ cells after differentiation, with or without APO and transforming growth factor beta1 (TGF-β1) inhibitor. C Representative images of tartrate-resistant acid phosphatase (TRAP)-stained osteoclast cultures. D TRAP5b and E TGF-β protein expression in the supernatant of osteoclast cultures. F Analysis of relative gene expression of Nfatc1 (major transcription factor in osteoclastogenesis) , Acp5 (TRAP critical for osteoclastogenesis), and Rank (a receptor specific to osteoclasts and progenitors) gene. Statistical analysis involved a one-way ANOVA followed by Tukey’s multiple post hoc test. Data are presented as mean ± SD, and significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001 ( n = 3–8).

    Article Snippet: After 3 days of cultivation, TGF-β1 protein expression (Invitrogen 88-50690-22) and TRAP5b activity (Bone TRAP, Immunodiagnostic Systems) were measured in the culture supernatant from BMM differentiated osteoclasts.

    Techniques: Fluorescence, Phagocytosis Assay, Derivative Assay, Labeling, Staining, Expressing, Gene Expression

    MiR‐106b inhibition decreased osteoclast numbers and suppressed osteoclast formation. A, Representative slices with TRAP staining. Scale bar: 50 μm. B, Oc.S/BS and C, N.Oc/BS were calculated. D, Representative immunohistochemical images of CTSK and MMP‐9. Scar bar: 100 μm. E,F, Semi‐quantitative analysis of CTSK and MMP‐9 expression. G, Serum levels of CTX and H, TRAP5b in each group were determined. n = 6 per group. * P < .05, ** P < .01, *** P < .001

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: MiR‐106b inhibition suppresses inflammatory bone destruction of wear debris‐induced periprosthetic osteolysis in rats

    doi: 10.1111/jcmm.15376

    Figure Lengend Snippet: MiR‐106b inhibition decreased osteoclast numbers and suppressed osteoclast formation. A, Representative slices with TRAP staining. Scale bar: 50 μm. B, Oc.S/BS and C, N.Oc/BS were calculated. D, Representative immunohistochemical images of CTSK and MMP‐9. Scar bar: 100 μm. E,F, Semi‐quantitative analysis of CTSK and MMP‐9 expression. G, Serum levels of CTX and H, TRAP5b in each group were determined. n = 6 per group. * P < .05, ** P < .01, *** P < .001

    Article Snippet: The serum levels of receptor activator of nuclear factor‐κ B ligand (RANKL), osteoprotegerin (OPG), osteocalcin (OCN), N‐terminal propeptide of type I procollagen (P1NP) and c‐terminal telopeptide of type I collagen (CTX), as well as the activity of TRAP5b, were assessed using specific ELISA kits (R&D Systems), according to the manufacturers’ instructions.

    Techniques: Inhibition, Staining, Immunohistochemical staining, Expressing